Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent_Vol.2(2)_pp.131-137
ISSN 1348-9615


Effect of collagen immobilized poly(ethylene-co-vinyl alcohol) to human pulp derived cell

Yoshihiro NISHITANI1, Ryo TAKAGI1, Naoki USHIRO1, Toshiyuki ITOTA1, Suong-Hyu HYON2, Sadami TSUTSUMI2 and Masahiro YOSHIYAMA1
1Department of Operative Dentistry, Okayama University Graduate School of Medicine and Dentistry
2Instutute for Frontier Medical Science, Kyoto University

The aim of this study was to investigate the morphology, growth and type I collagen expression of human pulp derived cells on the poly(ethylene-co-vinyl alcohol) (EVA) coated with collagen (+C) film. Cell morphology and growth curve for EVA+C group was similar to that for control group, which was cultured on the normal cell culture dish. However, for EVA group cell shape showed no extension and the growth was down-regulated. There was no difference in expression of type I collagen in the cultured cells among the three groups.
These results suggested that the immobilization of collagen to EVA facilitate an attachment and extension of human pulp derived cells on the EVA film without down regulation of type I collagen.

Keyword: collagen, poly(ethylene-co-vinyl alcohol), pulp cell, pulp capping