Recently, diabetic mellitus is dramatically increasing in our country. A diabetic often induces the serious periodontal disease and following tooth lost. Therefore, there is a necessity for considering the cell activity to the implant materials for the diabetic mellitus. In the present study, we examined the initial cell attachment to titanium plate by using the human gingival fibrobrasts derived from diabetic mellitus patients. The influence of type I collagen coating on titanium plate for the enhancement of initial cell attachment was evaluated. As a result, collagen coating to titanium plate was effective for the improvement of initial cell attachment for non-diabetic mellitus. On the contrary, for human gingival cell derived from diabetic mellitus under the high blood glucose condition of HbA1c 9%, collagen coating to titanium plate was not effective for the enhancement of initial cell attachment.