Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent Vol.5(2) pp.105-116
ISSN 1348-9615


Experimental Study of Sinus Augmentation by Controlled Release of Basic Fibroblast Growth Factor

Yasunori SAWADA1, Akishige HOKUGO2, 3, Shosuke MORITA2
1First Department of Oral and Maxillofacial Surgery, Graduate School of Dentisty, Osaka Dental University. 2First Department of Oral and Maxillofacial Surgery, Osaka dental University, 3Department of Biomaterials, Field of Tissue Engineering,Institute for Frontier Medical Sciences, Kyoto University

The purpose of this study was to evaluate the novel graft material for sinus augmentation by controlled release of basic fibroblast growth factor (bFGF) and biodegradable ceramics. We grafted following 4 materials into frontal sinus of beagle dogs. 1. β-tricalcium phosphate (β-TCP) and bFGF-incorporated gelatin hydrogel microspheres, 2. β-TCP and physiological saline-incorporated gelatin hydrogel microspheres, 3. β-TCP and bFGF solution, and 4. autologous bone. Augmented bone was evaluated by soft X-ray, histlogical examination and histomorphological examination at 4 and 12 weeks after implantation. When the β-TCP and bFGF-incorporated gelatin hydrogel microspheres were grafted, newly formed bone tissue volume was larger than other groups. This finding indicated that bFGF-incorporated gelatin hydrogel microspheres enables to effectively increase bone formation. We concluded that β-TCP and bFGF-incorporated gelatin hydrogel microspheres was a novel promising graft material for sinus augmentation, since it can be easily prepared and does not require a autologous bone harvest.

Keyword: basic fibroblast growth factor, gelatin hydrogel, β-tricalcium phosphate, drug delivery system