Journal of Oral Tissue Engineering

Quantitative Analyses of Osteogenic-differentiation-related Gene Expressions in Human Osteoblast-like Cells (SaOS-2) Cultured on Hydroxyapatite and Titanium

Masayuki TAIRA1, Wataru HATAKEYAMA2, Hidemichi KIHARA2, Hisatomo KONDO2, Kyosuke UEDA3 and Takayuki NARUSHIMA3

1Department of Biomedical Engineering, Iwate Medical University, Iwate, Japan
2Department of Prosthodontics and Oral Implantology, Iwate Medical University School of Dentistry, Iwate, Japan
3Department of Materials Processing, Graduate School of Engineering, Tohoku University, Sendai, Japan

J Oral Tissue Engin 2012;10(1):34-41

The purpose of this study was to examine osteogenic differentiation behaviors at gene expression levels of osteoblast-like cells (SaOS-2) when cultured on poly-styrene (PS), hydoroxyapatite (HAP) and titanium (Ti) for 8, 16 and 24 days, using six primer sets such as those for genes (mRNAs) of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), alkaline phosphatase (ALPL), type 1 collagen (COL1A1), osteopontin (SPP1), bone Sialo protein (IBSP) and osteocalcin pre-cursor (BGLAP). With respect to GAPDH control, ALPL and COL1A1; SPP1 and IBSP; and BGLAP were used as initial-stage, middle-to-late-stage, and last-stage osteogenic differentiation phenotype markers, respectively. Quantitative real-time RT-PCR analyses revealed that HAP most differentiated SaOS-2, followed by Ti while PS did least. It was re-confirmed that with respect to PS, HAP was an ex-cellent osteo-conductive material while Ti also exerted moderate osteo-conductive effects on SaOS-2 in the cell culture condition.

Key words: : SaOS-2, Osteogenic differentiation, Apatite, Titanium, Gene expression