Journal of Oral Tissue Engineering

Cell Viability of Mouse Cells by Exposure to the Dental Adhesives using Cell Recovery Test with 3D Culture

Koichi IMAI1, Kazuaki NAKAMURA2, Akito TANOUE2, Kazuhiko SUESE3, Tetsunari NISHIKAWA4, Akio TANAKA4, Chiaki WATANABE5, Hideo OHMUKAI5 and Hiromasa TAKASHIMA6

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
3Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
4Department of Oral Pathology, Osaka Dental University, Osaka, Japan
5Hatano Research. Institute, FDSC, Kanagawa, Japan
6Ina Research Inc., Nagano, Japan

J Oral Tissue Engin 2012;10(1):42-47

To examine the cytotoxicities of 11 commercially available dental adhesive products, mouse Balb/c 3T3 cells or cells derived from mouse uterine tissue origin were three-dimensionally cultured on a type I collagen gel to conduct a cell recovery test. The products showed significantly different cytotoxicities in both cells. The results demonstrated that 4-META products tend to be more cytotoxic than those containing Bis-GMA as the main ingredient, although no significant difference was noted between the cells. However, the manufacturer had not published the amount of each composition. Therefore, we could not fully understand the relationship between the composition and cell viability. All the products allowed cell recovery without any problem.

Key words: : cell viability, cell recovery, dental adheisive, Balb/c 3T3, mouse uterine cells