Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Comparison between the Two Endpoints of the Embryotoxicity Test using the Contracting Myocardial Cells and the Alkaline Phosphatase (ALP) Activity

 Koichi IMAI1, Kazuaki NAKAMURA2, Akito TANOUE2, Kazuhiko SUESE3, Fumiya OGAWA4 and Hiromasa TAKASHIMA5

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Department of Pharmacology, National Research Institute for
Child Health and Development, Tokyo, Japan
3Osaka Dental University,
School of Dental Technician and Hygienist, Osaka, Japan
4Department of Postgraduate Clinical Training,
Osaka Dental University, Osaka, Japan
5Ina Research Inc., Nagano, Japan
J Oral Tissue Engin 2012;10(2):82-88
SYNOPSIS
The Embryo Stem Cell Test (EST) using mouse ES cell differentiation is an in vitro embryotoxicity screening test protocol. This EST protocol requires a 10-day or longer culture because the differentiation of contracting myocardial cells. Therefore, we developed a novel assessment method using alkaline phosphatase (ALP) activity as an endpoint, and compared the results obtained with the EST and our methods regarding four kinds of dental monomer. Bis-GMA was found to be weakly embryotoxic by the EST method, but non-embryotoxic by our method using ALP activity. No difference was found in the results for the other monomers by these methods. In the future, more data should be collected to establish our method.

Key words: EST, alkaline phosphatase activity (ALP), embryotoxicity