Journal of Oral Tissue Engineering

Development of the Embryotoxicity Test using the Hybrid 3D Cultivation Technique of Human Hepatocytes and a Mouse ES cells - Use of Novel Feeder

Koichi IMAI1, Kazuaki NAKAMURA2, Akito TANOUE2, Kazuhiko SUESE3, Yoshitomo HONDA4, Fumiya OGAWA5, Chiaki WATANABE6, Hideo OHMUKAI6 and Hiromasa TAKASHIMA7
1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
3Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
4Institute of Dental Research,
5Department of Postgraduate Clinical Training, Osaka Dental University, Osaka, Japan
6Hatano Research. Institute, FDSC, Kanagawa, Japan
7Ina Research Inc., Nagano, Japan

J Oral Tissue Engin 2013;11(1):51-56

The teratogenicity of thalidomide was demonstrated by a modified Embryonic Stem Cell (EST) method using a hybrid culture with human hepatocytes. Six kinds of primary cells derived from the mouse uterus or oviduct were used as feeder layers. As a result, the beating rate due to EL-M3 cell differentiation was slightly lower for some feeder layers after the addition of thalidomide. However, there was no significant difference due to the addition of thalidomide. This may be explained by the presence of atypical cells in the feeder cells. The results will be further ana-lyzed.

Key words: human hepatocytes, uterus cells, oviduct cells, EL M3 cells, feeder cells, MEF