Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Evaluation of Bone Regeneration using Platelet-rich or Platelet-poor Plasma Combined with Autologous Mesenchymal Stem Cells

 Kenichirou YASUI1, Yoshiyuki ARIMA1, Li PEIQI1, Tomoko TOKUDA1, Eiko AZUMI1, Aki NISHIURA1, Tomohiko ITO2, Yoshiya HASHIMOTO3, Naoya UEMURA4, Shunsuke BABA4 and Naoyuki MATSUMOTO1
1Department of Orthodontics,
2First Department of Oral and Maxillofacial Surgery,
3Department of Biomaterials,
4Department of Oral Implantology,
Osaka Dental University, Osaka, Japan
J Oral Tissue Engin 2013;11(1):57-66
SYNOPSIS
In this study, mesenchymal stem cells (MSC) were combined with platelet-rich plasma (PRP) and platelet-poor plasma (PPP), to determine their effects on bone regeneration in mini-pig calvarial defects, which have similar bone regeneration rates as humans. MSCs were harvested from Clawn mini-pigs and cultured for 2 weeks using osseo-induction medium. MSCs were mixed with PRP and PPP as graft materials. Graft materials were transplanted into mini-pig calvarial defects created for 8, 12 and 18 weeks. By micro CT analysis, the volumetric density of new bone in the PRP + MSC and PPP + MSC groups were larger than that of the control group after 12 and 18 weeks transplantation, with the PRP + MSC group being the highest after 18 weeks. Histological analysis showed and new bone formation that was continuous along the original bone was observed in the PRP + MSC and PPP + MSC groups. This study demonstrated that the combination of PRP, PPP and MSCs were sufficiently adaptable for treating bone defects in mini-pigs.

Key words: bone regeneration, platelet rich plasma, platelet poor plasma, mesenchymal stem cell