Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
Embryotoxic Potential of the Dental Adhesives by the Cell Differentiation Culture with the Embryonic Stem Cell Test

Koichi IMAI1, Tetsunari NISHIKAWA2, Tomoharu OKAMURA2, Akio TANAKA2,
Kazuhiko SUESE3, 4, Yoshitomo HONDA5, Hirofumi SAWAI6 and Hiromasa TAKASHIMA7

1Department of Biomaterials, 2Department of Oral Pathology,
3Osaka Dental University, School of Dental Technician and Hygienist,
4Department of Esthetic Dentistry, 5Institute of Dental Research,
6Department of Internal Medicine, Osaka Dental University, Osaka, Japan,
7Ina Research Inc., Nagano, Japan


J Oral Tissue Engin 2013;11(2):148-153

SYNOPSIS
Dental adhesive monomers are extensively used in clinical dentistry and are indispensable for repairing hard tissue. Products commercially available in Japan have passed a number of biological safety evaluation tests, including a cytotoxicity test, and are included as dentin adhesives in managed-care equipment. However, these embryotoxicity risks of each dental adhesive are unknown. Thus, an in vitro embryotoxicity test was conducted using the Embryo Stem Test (EST). As a result, all products were within the range of "non-embryotoxicity", while some products were near "weak embryotoxicity". The products containing Bis-GMA tended to show lower IC50 and ID50 values, with some exceptions. In contrast, those containing MMA compositions tended to have few effects on cell differentiation. These dental adhesive should be investigated further regarding their biological properties.

Key words: EST, dental adhesive monomer, embryotoxicity, Bis-GMA, MMA