Journal of Oral Tissue Engineering

The Effect of Artificial Recombinant Bone Morphogenetic Protein 2 with Titanium-binding Peptide on a Ti Graft in Vivo

Eitoyo KOKUBU1, Katsutoshi KOKUBUN2, Kenichi MATSUZAKA2, Kenji KASHIWAGI3, Kiyotaka SHIBA3, Takashi INOUE2, Kazuyuki ISHIHARA1 and Masao YOSHINARI4
1Oral Health Science Center, and Department of Microbiology, Tokyo Dental College, Tokyo, Japan
2Oral Health Science Center, and Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan
3Division of Protein Engineering, The Cancer Institute of the Japanese foundation for Cancer Research, Tokyo, Japan
4Oral Health Science Center, and Department of Dental Materials Science, Tokyo Dental College, Tokyo, Japan

J Oral Tissue Engin 2014;12(1):1-7

Surface modification of titanium (Ti) influences cell behavior, and immobilization of bone morphogenetic protein (BMP)-2 on a Ti surface cells to differentiate into osteogenic cells. This study aimed to evaluate the effect of an artificial fusion protein (AFP) comprising a modified BMP-2 and Ti-binding peptide (TBP) motif (AFP-TBP-BMP-2) on osteogenesis of rat abdominal cells on Ti surface in vivo. Ti plates were dipped in three different mixtures of collagen gel: collagen gel only, collagen gel with BMP-2, and collagen gel with AFP-TBP-BMP-2. The treated Ti plates were then implanted into rat abdominal muscles. One and 2 weeks after implantation, these plates were histologically observed. The Ti plate with collagen gel and AFP-TBP-BMP-2 produced cartilage in the muscle at 1 week, and bone-like hard tissue was observed at 2 weeks. These results suggest that the application of collagen gel with AFP-TBP-BMP-2 accelerates osteogenesis in vivo.

Key words: titanium-binding peptide, bone morphogenetic protein-2, artificial protein