Journal of Oral Tissue Engineering

Effects of Novel Synthetic Peptide during Early Wound Healing of Periodontal Artificial Defects in Rats

Momoe NANDO1, Kazuya TOMINAGA2, Tsurayuki TAKAHASHI3, Akio TANAKA2 and Makoto UMEDA3
1 Graduate School of Dentistry (Periodontology),
2 Department of Oral Pathology, 3 Department of Periodontology,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2014;12(2):69-79

To clarify the function of novel synthetic peptide with an amino acid sequence the same as a partial sequence of amelogenin gene exon 5, we applied them to periodontal tissue defects artificially prepared in the rat molar region. Sections of the applied regions were prepared 3, 5, 7, and 14 days after surgery and histopathologically investigated. In addition, type III collagen and von Willebrand factor were immunohistochemically observed. Epithelial cell growth was promoted for 3 days after surgery and then inhibited thereafter. Fibroblast growth was advanced, type III collagen decreased in the early phase, and the ratio of blood vessels significantly increased, showing the promotion of vascularization. The novel synthetic peptide seems to promote the wound-healing process, inducing periodontal tissue regeneration in the early phase.

Key words: novel synthetic peptide, early wound healing, type III collagen, von Willebrand factor