Journal of Oral Tissue Engineering

In Vitro Study of Cell Differentiation by Two Type Mouse Embryo Stem Cells on the Dental Adhesives

Koichi IMAI1, Kazuhiko SUESE2,3, Yoshitomo HONDA4, Tsubasa SHIRAI5, Fumiya OGAWA6, Hirofumi SAWAI7, Muneyasu SHIDA8 and Hiromasa TAKASHIMA9
1Department of Biomaterials, 2Department of Esthetic Dentistry,
3Osaka Dental University, School of Dental Technician and Hygienist,
4Institute of Dental Research, 5Graduate School of Dentistry (Biomaterials),
6Department of Postgraduate Clinical Training,
7Department of Internal Medicine, 8Department of Endodontics,
Osaka Dental University, Osaka, Japan
9Ina Research Inc., Nagano, Japan

J Oral Tissue Engin 2014;12(2):85-92

Embryonic Stem Cell Test (EST) is an in vitro embryotoxicity screening test to predict human developmental toxicity, mainly used in Europe and the United States. In the present study, EL-M3 and ES-R1-EGFP B2/EGFP cell lines were used, instead of the mouse ES-D3 cell line that is used as an indicator of cell differentiation, to compare the developmental toxicities of 11 dental adhesives with those obtained with ES-D3 cells. The results were comparable between EL-M3 and ES-D3 cell lines. Specifically, all the products fall into the category of "non- embryotoxicity," although the results widely varied. Three-dimensional culture with glass fibers could also be employed for the experiment.

Key words: dental adhesive monomer, embryotoxicity, EL-M3 cells, ES-R1-EGFP B2/EGFP cells