Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
Application of Porous Materials to Three-dimensional Tissue Culture in Capillary Formation in Vitro

Tomoharu OKAMURA1, Tetsunari NISHIKAWA1, Masahiro WATO1, Kazuya TOMINAGA1, Hirohito KATO1, Koichi IMAI2 and Akio TANAKA1
1Department of Oral Pathology, 2Department of Biomaterials,
Osaka Dental University, Osaka, Japan



J Oral Tissue Engin 2014;12(2):93-99

SYNOPSIS
The purpose of the present study is that attempting three-dimensional tissue culture of capillaries in vitro, using three kinds of porous scaffolds, coral, ceramic bone, and pumice stone. The proliferation of cells was enhanced time-dependently in the experimental group. 7, 14 and 21 days after addition of particles, the cell proliferation of the experimental group was significantly higher than that of the control group. In the surrounding region from the added pumice stone, capillary formation was low. Much cell proliferation and capillary formation was observed three-dimensionally around the coral and ceramic bone particles. In the coral additional group, the largest capillary width was observed in the anastomosis region of capillaries.

Key words: porous material, scaffold, capillary, three-dimension