Journal of Oral Tissue Engineering

Study of Hybrid Culture Technique using Human Hepatocytes and Mouse ES Cells by Three-dimensional Culture Scaffolds

Koichi IMAI1, Tsubasa SHIRAI1, Yoshitomo HONDA2, Tetsunari NISHIKAWA3, Tomoharu OKAMURA3, Akio TANAKA3, Fumiya OGAWA4, Hirofumi SAWAI5, Muneyasu SHIDA6 and Kazuhiko SUESE7
1Department of Biomaterials, 2Institute of Dental Research, 3Department of Oral Pathology,
4Department of Postgraduate Clinical Training, 5Department of Internal Medicine, 6Department of Endodontics,
7Department of Esthetic Dentistry, Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2014;12(3):121-126

An in vitro embryonic stem cell test allows the screening of potential reproductive and developmental toxicities, but does not reflect human metabolic activators. Thus, a novel test for the hybrid cultures of hepatocytes and mouse ES cells should be developed. To develop procedures for the hybrid cultures of three kinds of hepatocyte and mouse ES cells, basic structures for hybrid cultures and two scaffolds for three-dimensional cultures were examined to determine optimal experimental conditions.
In the comparison of basic structures for hybrid cultures, the incidence was slightly higher with hepatocytes placed outside the inter cell and embryo bodies (EBs) inside. As a result of the comparison of the scaffolds, glass fiber showed a slightly higher incidence than collagen gel scaffolds.

Key words: EST, three-dimensional culture, ES cells, hepatocytes, scaffold