To develop highly biologically safe dental gold-silver-palladium alloy compositions, the surfaces of 2 commercially available alloys were scraped with waterproof abrasive paper and corroded with hydrochloric acid, sulfuric acid, and artificial saliva, and the embryotoxicity of the corroded products was investigated. The embryotoxicity was evaluated based on the rate of differentiation of mouse ES-D3 cells to the mouse myocardial cells used in Embryo Stem Cell Test (EST) protocol and alkaline phosphatase (ALP) activity. Compared with that of the negative control without surface corrosion, the cell differentiation rate slightly decreased. The results of ALP activity were similar.
Dental gold-silver-palladium alloys are the most frequently used dental alloys in Japan. It was clarified that it is necessary to analyze data on various gold-silver-palladium alloy prototypes and investigate alloy compositions with a low risk of embryotoxicity.
Key words: ES cells, gold-silver-palladium alloy, corrosion, differentiation, artificial saliva
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