Journal of Oral Tissue Engineering

Study of Embryotoxicity of Commercial Dental Gold-silver-palladium Alloy by Corrosion using Acid and Artificial Saliva

Koichi IMAI1, Yoshihiro NISHITANI2, Tomohiro HOSHIKA3, Mari AKIYAMA1, Shosuke MORITA4, Tomio ISEKI4, Hiroaki YOSHIDA4, Kazuhiro MATSUMOTO4, Isao TAMURA5, Muneyasu SHIDA6, Hirofumi SAWAI7, Tadashi OHKUBO7 and Kazuhiko SUESE8

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Department of Restrative Dentistry and Endodontology,
Kagoshima University, Kagoshima, Japan
3Department of Operative Dentistry,
Okayama University Hospital, Okayama, Japan
4First Department of Oral and Maxillofacial Surgery,
5Department of Oral Anatomy, 6Department of Endodontics,
7Department of Internal Medicine, 8Department of Esthetic Dentistry,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2015; 13(2): 77-84

To develop highly biologically safe dental gold-silver-palladium alloy compositions, the surfaces of 2 commercially available alloys were scraped with waterproof abrasive paper and corroded with hydrochloric acid, sulfuric acid, and artificial saliva, and the embryotoxicity of the corroded products was investigated. The embryotoxicity was evaluated based on the rate of differentiation of mouse ES-D3 cells to the mouse myocardial cells used in Embryo Stem Cell Test (EST) protocol and alkaline phosphatase (ALP) activity. Compared with that of the negative control without surface corrosion, the cell differentiation rate slightly decreased. The results of ALP activity were similar.
Dental gold-silver-palladium alloys are the most frequently used dental alloys in Japan. It was clarified that it is necessary to analyze data on various gold-silver-palladium alloy prototypes and investigate alloy compositions with a low risk of embryotoxicity.

Key words: ES cells, gold-silver-palladium alloy, corrosion, differentiation, artificial saliva

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