Journal of Oral Tissue Engineering


Influence on the Long-term Differentiation Culture of ES-D3 Cells with String-like Collagen Scaffolds Derived from Tilapia Scales

Koichi IMAI1, Tetsunari NISHIKAWA2, 3, Shosuke MORITA4,
Tomio ISEKI4, Hiroaki YOSHIDA4, Kazuhiro MATSUMOTO4,
Muneyasu SHIDA5, Fumiya OGAWA6 and Kazuhiko SUESE7

1 Department of Biomaterials, 2 Department of Oral Pathology
3 Department of Innovation in Dental Education
4 First Department of Oral and Maxillofacial Surgery,
5 Department of Endodontics, 6 Department of Postgraduate Clinical Training,
7 Department of Esthetic Dentistry,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2016; 13(3): 125-130

In the field of regenerative medicine, tissues and organs differentiated from iPS cells are implanted into the body. For this purpose, scaffolds using collagen derived from fish, evolutionarily distant from humans, are safer than those using mammalian (porcine) collagen. In the present study, the differentiation of mouse-derived ES cells was examined using string-like porous collagen derived from tilapia that inhabits tropical and subtropical regions. Cell differentiation into bone or cartilage requires 30-day culture. Whether or not the string-like collagen could withstand the long-term culture was examined, demonstrating that 86% of the collagen retained a gel form during the culture without any effect on ES cell differentiation.

Key words: ES cells, marine collagen, porous collagen, scaffold, Tilapia

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