Journal of Oral Tissue Engineering


Study of Pluripotency and Activation of Totipotency in Human Adipose-derived Stem Cells

Shunji IZAWA, Kozo YAMAJI, Akihito YOKOYAMA,
Kumiko Matsuzaki, Naoko OHARA
and Masahiro YOSHIYAMA

Department of Operative Dentistry, Okayama University
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,
Okayama, Japan

J Oral Tissue Engin 2016; 13(3): 131-135

Tissue regeneration is the important issue in the field of operative dentistry. In the previous study, we demonstrated that TNFα activates the potential for totipotency in human bone marrow mesenchymal stem cells (hMSCs) and leads to the regeneration of target cells or tissues along with the appropriate differentiating factor such as Emd-Gel. In this study, we apply the transcription factor set of TNF-α and Amelogenin to the human adipose-derived stem cells (hASCs) and our results indicate that this transcription factor set differentiate hASCs into odontblasts simillary and suggest that TNFα activates potential totipotency of hASCs and the activation can lead to the regeneration of target cells or tissues

Key words: human adipose-derived stem cells (hASCs), TNFâ┐, Amelogenin, differentiation, totipotency

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