Journal of Oral Tissue Engineering

Cytokines Levels in Peri-Implant Crevicular Fluid in the Intial Stages of Dental Implant Treatments - A Preliminary Clinical Study

Kuppusamy MAHESWARI, Jia HAO, Zayar LIN, Hidemi NAKATA, Shohei KASUGAI, and Shinji KURODA

Oral Implantogy and Regenerative Dental Medicine,
Department of Oral Health Sciences, Graduate School,
Tokyo Medical and Dental University, Tokyo, Japan

J Oral Tissue Engin 2016; 14(1): 2 - 8.

Objectives:The purpose of the present study is to measure and monitor the lev-els of potential pro- inflammatory cytokine interleukin-1beta (IL-1β) Tumor necrosis factor-alpha (TNF-α) in Peri-implant crevicular fluid (PICF) for the level of activity of inflammatory processes during the various stages of implant delivery system.
Materials and Methods:A total of 12 dental implants were placed in nine partially edentulous patients (mean age of 60 years). External Branemark implants with anodic oxidized surface were installed and submerged in all the patients. The healing abutments were delivered at the second surgery and they were removed more than three times till the final prosthesis delivery. At different time points during the treatment, peri-implant cervicular fluid (PICF) was collected and pro-inflammatory cytokine (IL-1β and TNF-α) were measured by enzyme-linked immunosorbent assay (ELISA). The clinical indices were also recorded at every time point.
Results:The pro-inflammatory cytokine levels were different during different time points. Increased level of IL-1β levels during various stages of implant delivery. All the implants were Osseo integrated.
Conclusion:The findings of the present study indicated that level of IL-1beta, may be an important biomarker in addition to clinical findings in measuring the health condition of the peri-implant tissues.

Key words: Cytokines, Peri-implant cervicular fluid, Inflammation, wound healing