Journal of Oral Tissue Engineering

Histological Evaluation of Alveolar Bone Ridge Augmented with Platelet-rich Fibrin and Artificial Bone

Akihiro SUNANO1, Nozomu FUKUI1, Miwa KANOU1, Yoshihiro KIMURA1, Yoichiro NAKAJIMA1, Yuichi ITO1, Yasunori ARIYOSHI1,2, Haruhiko TERAI1 , Peter K. MOY3, and Takaaki UENO1

1Division of Medicine for the Function and Morphology of Sensory Organs,
Department of Dentistry and Oral Surgery,
Osaka Medical College, Osaka, Japan
2Clinic of Oral and Maxillofacial Surgery,
Hirakata Municipal Hospital, Osaka, Japan
3Surgical Implant Dentistry, University of California,
Los Angeles, CA, USA

J Oral Tissue Engin 2016; 14(1): 15 - 20.

Platelet-rich fibrin (PRF) includes various growth factors, and it has been reported to stimulate hard and soft tissue healing. However, there have been very few articles discussing its histological features. In the present report, we observed core samples harvested from human maxilla bone augmented with artificial bone combined with PRF for sinus floor augmentation. The patient underwent sinus floor augmentation using artificial bone combined with PRF. When the second implant surgery was performed, cylindrical bone core samples were harvested from the augmented area. Samples were prepared using the non-decalcifying fresh-frozen section technique and were observed with a microscope. Histologically, we observed soft and hard tissue throughout the region observed. In the hard tissue area, new bone formation around the artificial bone particles and new bone invading the artificial bone were observed. The core sample harvested from maxilla augmented by artificial bone combined with PRF showed histologically active new bone formation.

Key words: Platelet rich fibrin, bone augmentation, histological evaluation, dental implant