Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
Effects of High-frequency Electromagnetic Wave Stimulation on Bone Repair in Rat Calvaria Defects

Takahito SATO1, Tsutomu SUGAYA1, Megumi KUDO1, Mitsunori NAKATANI1, Nagako MAEDA1, Masamitsu KAWANAMI1, and Toshihiko TOMINAGA2

1Department of Periodontology and Endodontology, Division of Oral Health Science, Hokkaido University
Graduate School of Dental Medicine, Sapporo, Japan
2Tominaga Dental Clinic, Naruto, Japan



J Oral Tissue Engin 2016; 14(1): 59 - 64.

SYNOPSIS
High-frequency electromagnetic wave stimulation induces proliferation and differentiation of osteoblasts. The aim of the study is to histopathologically evaluate the effects of high-frequency electromagnetic waves on bone formation.
Bone defects with a diameter of 4.8 mm were created on the left and right sides of the calvarias of Wistar rats. The active electrode was placed in the left defect and high-frequency electromagnetic stimulus was applied for 1 s and repeated five times. This was performed twice a week for 2 weeks. The right defects were not stimulated (control group). After 8 weeks, histological specimens were prepared by hematoxylin and eosin (HE) staining, and the rate of bone formation was measured.
The rate of bone formation was significantly larger in the experimental group than in the control group. These results indicate that high-frequency electromag-netic wave stimulation enhances bone formation in rat calvarial defects.

Key words: high-frequency electromagnetic waves, bone formation, calvarial defect