Journal of Oral Tissue Engineering

Healing Mechanism Surrounding Transplanted Bone Using Transgenic Mice Expressing Red Fluorescent Protein in Vivo

Manabu INOUE1, Keishi OTSU2, Masato OHTSUKA3, Kyoko TAKAFUJI1, Hidemitsu HARADA2, Akira ISHISAKI4, and Hisatomo KONDO1

1Department of Prosthodontics and Oral Implantology,
School of Dentistry, Iwate Medical University, Morioka, Japan
2Division of Developmental Biology & Regenerative Medicine,
Department of Anatomy,
Iwate Medical University, Morioka, Japan
3Department of Molecular Life Science,
Division of Basic Medical Science and Molecular Medicine,
Tokai University School of Medicine, Kanagawa, Japan
4Division of Cellular Biosignal Sciences, Department of Biochemistry,
Iwate Medical University, Morioka, Japan

J Oral Tissue Engin 2016; 14(2): 74 - 82.

In recent years, Use of dental implants is becoming more widespread; however, not all patients can receive implant treatment without bone grafting. Bone grafting is frequently performed in implant treatments, and graft materials include autogenous bone, allografts, xenografts, and alloplasts. The healing mechanism of the autogenous bone graft is not well known; therefore, this study examines the healing mechanism surrounding grafted bone, at both cellular and molecular levels, employing tissue derived from transgenic mice expressing red fluorescent protein in vivo to monitor cellular kinetics in situ and to elucidate the healing mechanism. Results of our study suggested that mineralized tissue regeneration was induced by the transplanted bone representing autogenous bone graft but occurred from the margin of the existing bone.

Key words: bone, transplant, tdTomato, regeneration, cell kinetics