Journal of Oral Tissue Engineering
Stability of Platelet-rich Fibrin in Vivo: Histological Study in Rats
Yu YAMASHITA, Kang CHEN, Shinji KURODA, and Shohei KASUGAI
Department of Oral Implantology and Regenerative Dental Medicine,
Graduate School of Medical and Dental Sciences,
Tokyo Medical and Dental University, Tokyo, Japan
J Oral Tissue Engin 2016; 14(2): 83 - 90.
Purpose: Platelet-Rich Fibrin (PRF) is a product prepared from the patient's peripheral blood, containing several growth factors and fibrins, potentially stimulating tissue regeneration. The purpose of the present study is to examine the stability of PRF in vivo.
Methods: Under anesthesia, a small amount of blood was collected into a small glass tube from the superficial femoral vein of a Wistar rat and PRF was immediately prepared after spinning down. Then, the PRF was transplanted and stitched to the back muscle of the same animal. The animals were sacrificed at 1, 3, 5, 7, 14, 21 and 28 days. The transplanted region was examined histologically and immuno-histochemically with an antibody against rat PDGF.
Results: Histological and immunohistochemical results demonstrated that the PRF became small and sparse at 21 days, almost disappearing at 28 days.
Conclusion: Although PRF can potentially stimulate tissue regeneration, its deg-radation time should be considered in its clinical applications. It is also obvious that PRF alone cannot work as a scaffold or a barrier membrane for maintaining the space of regeneration.