Journal of Oral Tissue Engineering

Prediction of Embryotoxicity by Four Kinds of Fluorine Compounds Based on Embryonic Stem Cell Test (EST) Protocol

Koichi IMAI1, Hiroshi OSHIMA1, Yoshiya HASHIMOTO1, Mari AKIYAMA1, Masayuki ZENNYU1, Takamitsu YOSHIDA1, Tetsunari NISHIKAWA2, Kazuya MASUNO2, and Hidenori MATSUMOTO2

1Department of Biomaterials, 2Department of Innovation in Dental Education,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2016; 14(2): 91 - 97.

The safety of fluoride use to prevent dental caries has been controversial. To clarify the risk of embryotoxicity of fluoride influencing human fetuses/newborns, we investigated the embryotoxicity levels of 4 fluorides (NaF, KF, SnF2, and CuF2) using the EST method, which is an in vitro embryotoxicity screening test devel-oped by Spielmann et al. in 1997. Of the fluorides, NaF and SnF2 are used to prevent dental caries in Japan. All substances were "non-embryotoxic", but SnF2 and CuF2 were close to the borderline of "weak embryotoxic". Since many young people use fluoride to prevent dental caries, further investigation of the em-bryotoxicity of fluorine is desired.

Key words: embryotoxicity, fluoride, NaF, SnF2, cell differentiation