Journal of Oral Tissue Engineering

Attachment of Mouse Osteoblast-like Cells onto Fibronectin- or Albumin-immobilized Poly (Lactic Acid-ε-Caprolactone) Films

Megumi FUSE1, Tohru HAYAKAWA2, Tomomi HASHIZUME-TAKIZAWA3, Hidenori SUZUKI1, Hiroaki WAKAI1, Makoto NAKANISHI1, Yoshimine NIKI1, Hong Sung-Moon1, Katsuhiro ASAKA1, Tomoko KURITA-OCHIAI3 and Masahiko FUKUMOTO1

1Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan
2Department of Dental Engineering, Tsurumi University School of Dental Medicine, Yokohama, Japan
3Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan

J Oral Tissue Engin 2017; 15(1): 3-10.

In this study, two different proteins, fibronectin (FN) or albumin (Alb), were immobilized onto poly (lactic acid-ε-caprolactone) (PLCL) films. FN is a cell adhesion protein, whereas Alb is a cell adhesion-inhibiting protein.The amount of immobi-lized FN on the PLCL film was significantly greater than that for Alb (p < 0.05). The number of MC3T3-E1 cells that adhered to FN-PLCL films was larger than that for Alb-PLCL films (p < 0.05) in a 90-min cell assay. After 3 days of culture, cells appeared more flattened on FN-PLCL films, and small lamellipodia and filopodia were observed. These results indicate that FN and Alb-immobilized onto PLCL films retained their cell adhesion-promoting and cell adhesion-inhibiting properties, respectively.

Key words: Poly (Lactic Acid-ε-Caprolactone) film, Fibronectin, Albumin, Osteoblast-like cell, Alkali treatment

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