Journal of Oral Tissue Engineering

Ligation Period Required to Induce Periodontitis in Mice: Analysis with Micro-computed Tomography

Boosana KABOOSAYA1,3, Lia Kartika WULANSARI1, Trang Nguyen V.N.1, Kazuhiro AOKI2, and Shohei KASUGAI1

1Department of Oral Implantology and Regenerative Dental Medicine, 2Department of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences
Tokyo Medical and Dental University, Tokyo, Japan
3Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand

J Oral Tissue Engin 2017; 15(1): 25-34.

Placing a ligature around the mouse molar have been widely used in experimental periodontitis to investigate disease mechanisms and novel treatments. However, the period of ligation was different in the previous studies: From 1 week to 3 weeks. The aim of the present study is to verify the proper period of ligation to induce periodontitis in mice. The ligature was placed around the upper second molars of 18 male C57BL/6 mice, 8 weeks old, and the animals were sacrificed at 1 and 3 weeks. Then, the maxillae were analyzed with micro-computed tomography. The amount of alveolar bone loss at 1 week was statistically similar to the one at 3 weeks. The present results suggested that the ligation for 1 week is enough to induce periodontitis in mice.

Key words: ligature-induced periodontitis, mouse, period, alveolar bone loss