Journal of Oral Tissue Engineering

Influence of Fluoride Contamination on Titanium Surface on Cell Viability and Cell Differentiation of ES-D3 Cells

Koichi IMAI1, Masayuki ZENNYU1, Takamitsu YOSHIDA1, Yoshiaki ONO2, Shunji KUMABE3, and Tetsunari NISHIKAWA4

1Department of Biomaterials, 2Department of Geriatric Dentistry,
3Department of Oral Anatomy, 4Department of Innovation in Dental Education,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2017; 15(1): 35-40.

Developmental toxicity of chemical substances seriously impairs normal birth of human newborns. Corrosion resistance of titanium used in implant fixtures extremely decreases in the presence of fluorine ion produced by acidification. To our knowledge, cytotoxicity and developmental toxicity of corrosion products formed by corrosion of the titanium surface by fluoride have not been reported. Thus, we investigated differ-entiation and proliferation of ES-D3 cells, which is a parameter employed in the EST method. When the surfaces of JIS Class 1 and 2 titanium plates were corroded with hydrofluoric acid solution, cell differentiation and proliferation were affected. It was clarified that although this finding does not directly lead to clinical developmental toxicity, it is also necessary to investigate corrosion of titanium alloys used in implant fixtures.

Key words: Fluoride contamination, Ti, Cell viability, Cell differentiation, ES cells