Journal of Oral Tissue Engineering

Effects of Montipora digitata Exoskeleton- derived Aragonite Particles on Human Fibroblasts for Cell Proliferation and Collagen Production in Vitro

Tomoharu OKAMURA1, Tomoki TAKEUCHI1, Shuta HONDA1, Kazuya TOMINAGA1, Kaori NARUSE2, Shosuke MORITA2, Koichi IMAI3, Kazuya MASUNO4, Yoshiaki ONO5, Tetsunari NISHIKAWA4, and Akio TANAKA6

1Department of Oral Pathology, 2First Department of Oral and Maxillofacial Surgery,
3Department of Biomaterials, 4Department of Innovations in Dental Education,
5Department of Geriatric Dentistry, 6Department of Pathology,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2017; 15(1): 41-48.

In regenerative dental therapy, the development of bone scaffold materials to enable augmentation of bone for dental implant and prosthesis treatment is important. Montipora digitata exoskeleton-derived aragonite particles are porous, bioresorbable bone scaffold and applied to bone defects as natural materials. In this bone scaffold material, it is necessary to form collagen fibers which are the extracellular matrix of particles derived from Montipora digitata skeleton capillary vessels and newly formed bone. Therefore, we cultured fibroblast cells and added the particles in order to investigate the effect of cell proliferation ability and production of collagen fibers. In this study, it was observed that cultured fibroblasts were enhanced cell proliferation and collagen fiber production by adding aragonite particles.

Key words: Coral, Fibroblast, Collagen