Journal of Oral Tissue Engineering

Induction of Mesenchymal Stem Cells-like Cells Derived from Human Gingival iPS Cells into Osteoblast-like Cells

Yuji NAKAYAMA1, Yoshiya HASHIMOTO2, Yoshitomo HONDA3, and Naoyuki MATSUMOTO1

1Department of Orthodontics, 2Department of Biomaterials,
3Institute of Dental Research, Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2017; 15(2): 85-94.

Mesenchymal stem cells (MSCs)@have limited capacity to proliferate in vitro, making it very difficult to acquire sufficient cell numbers for implantation. The objective of this study was to induce MSC-like cells (MSLCs) derived from human gingival induced pluripotent stem (HG-iPSCs) into osteoblast-like cells. We derived MSLCs from HG-iPSCs under feeder-free conditions. MSLCs expressing MSC-specific markers treated with osteoblast differentiation factor expressed osteoblast-specific mRNA and protein level when cultured with or without bone morphogenetic protein (BMP). BMP-2 had a positive effect on the expression of early indicators of osteogenic differentiation. However, the medium without BMP-2 promoted calcium deposition, which occurs at the terminal stage of osteogenic differentiation. MSLCs are likely to have higher proliferative capacity, and hence, in vitro-differentiated osteoblast-like cells and appropriate scaffolds can be used for bone tissue engineering.

Key words: mesenchymal stem cells-like cells, mesenchymal stem cells, human gingival, iPS cells, osteoblast-like cells