This study compared bone regeneration ability following non-demineralized dentin granule transplantation and autogenous bone grafting. A critical-sized bone defect was created at the center of the calvaria in rats, and autologous bone and dentin were transplanted. All specimens were evaluated microradiographically and histologically. Computed tomography (CT) analysis revealed no difference in the amount of hard tissue between the dentin-transplanted group and the autologous bone-grafted group 8 weeks after surgery. Tissue analysis revealed that the defect was almost entirely replaced with new bone in the autogastric bone-grafted group 8 weeks after surgery. About half of the dentin granules remained in the dentin-transplanted group, and the rest were replaced with autologous bone. These results suggest that dentin was slowly replaced by new bone while securing the space at the transplantation site, even after a long period of transplantation.
Key words: bone tissue engineering, autologous bone graft, dentin graft, CT analysis, rat calvarial defect
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