We prepared DNA/cationic polylysine complexes, and examined their biodegradation behavior and histopathological responses for use as a scaffold material for bone regeneration. For the cationic polylysine, two types of isomeric polylysine, D-polylysine (DNA/D-lysine complex) and L-polylysine (DNA/L-lysine complex) were used. A water-insoluble white powder of the complexes with a porous structure was obtained. The yields of the DNA/D-lysine complexes and DNA/L-lysine complexes were 63.8% and 64.5%, respectively. There were mild tissue responses after a disk comprising either complex was implanted into the subcutaneous tissue of rats. The biodegradation rate of the DNA/L-lysine complexes was greater than that of the DNA/D-lysine complexes. This study suggested that DNA/cationic polylysine complexes have the potential for controlled biodegradation using satisfactory combinations of isomeric polylysine.
Key words: DNA, optical isomer, lysine, biodegradation, biocompatibility
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