Journal of Oral Tissue Engineering

Preparation and Biodegradation Properties of DNA/poly-lysine Complexes by FE-SEM

Kimiko OHGI1, Ayako MATSUMOTO3,
and Ryuji SAKAGAMI1

1Department of Odontology, Periodontology Section,
2Department of Dental Engineering, Biomaterials Section,
3Department of Oral Rehabilitation, Oral Implantology Section,
Fukuoka Dental College, Fukuoka, Japan

J Oral Tissue Engin 2019; 17(1): 1-8.

We prepared DNA/cationic polylysine complexes, and examined their biodegradation behavior and histopathological responses for use as a scaffold material for bone regeneration. For the cationic polylysine, two types of isomeric polylysine, D-polylysine (DNA/D-lysine complex) and L-polylysine (DNA/L-lysine complex) were used. A water-insoluble white powder of the complexes with a porous structure was obtained. The yields of the DNA/D-lysine complexes and DNA/L-lysine complexes were 63.8% and 64.5%, respectively. There were mild tissue responses after a disk comprising either complex was implanted into the subcutaneous tissue of rats. The biodegradation rate of the DNA/L-lysine complexes was greater than that of the DNA/D-lysine complexes. This study suggested that DNA/cationic polylysine complexes have the potential for controlled biodegradation using satisfactory combinations of isomeric polylysine.

Key words: DNA, optical isomer, lysine, biodegradation, biocompatibility

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