A bone defect was created at the center of a rat calvaria. Autologous bone and dentinal granules with autologous bone were transplanted. All specimens were evaluated microradiographically and histologically. CT revealed no difference in the amount of hard tissue between the autologous bone and dentinal granules with autologous bone group 4, 6, and 8 weeks post-surgery. Tissue analysis of the dentinal granules with autologous bone group, 8 weeks post-surgery, revealed that the transplanted autologous bone granules were entirely replaced with the new bone. Meanwhile, about a quarter of the added dentinal granules remained as dentinal granules with autologous bone, and the rest were replaced with autologous bone. In addition, the diameter of the dentin granules decreased with time. Therefore, the addition of dentinal granules to autogenous bone grafts does not inhibit replacement of the new bone and imparts bone regeneration ability to autologous bone grafts for a prolonged period of time.
Key words: bone tissue engineering, autologous bone graft, dentin graft, CT analysis, rat calvarial defect
All documents in this paper