Journal of Oral Tissue Engineering

Identification of Novel Genes Related to Tooth Morphogenesis

Masato NAKAGAWA1,2, Makoto TAKEO1, Miho OGAWA1, Yohei YUGE3, Masato YASUKAWA3, Tomomi TOKITA3, Kentaro ISHIDA4,5, Etsuko IKEDA1,2, Tadaaki KIRITA2, and Takashi TSUJI1

1RIKEN Center for Biosystems Dynamics Research, Hyogo, Japan
2Department of Oral and Maxillofacial Surgery, Nara Medical University, Nara, Japan
3Department of Biological Science and Technology, Graduate School of Inductrial Science and Technology,
Tokyo University of Science, Chiba, Japan
4Research Institute for Science and Technology, Tokyo University of Science, Chiba, Japan
5Depertment of Developmental Biology, Graduate School of Medicine, Chiba University, Chiba, Japan

J Oral Tissue Engin 2020; 18(2): 41-54.

Tooth development is controlled by body plan during the fetal period, the generation of teeth from tooth germ is induced by the epithelial-mesenchymal interaction. Spatiotemporal regulation of tooth morphogenesis is supported by gene expression. Although many of the genes involved in tooth development are known, the molecular mechanism underlying tooth morphogenesis is not completely understood. For a comprehensive understanding of tooth development, the elucidation of unknown genes is necessary. In this study, to identify unknown genes involved in tooth development, we performed genome-wide analysis at each stage of tooth development and identified 17 genes with high levels of expression and large changes in expression. In addition, we performed qPCR and in situ hybridization analyses to elucidate the spatiotemporal regulation, such as the regulation that occurs around or in the entire tooth germ, enamel knots, epithelium, and mesenchyme. These results show that these characteristic genes may play important roles in each time period or region of tooth development, and the elucidation of the functions of these genes will lead to an integrated understanding of the process of tooth development.

Key words: development, gene ontology, microarray analysis, qPCR, in situ hybridization

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