Journal of Oral Tissue Engineering

The Role of Syndecans in the Human Dental Pulp

Kenji TSUDA1, Aiko KAMADA2, Yoshihiro YOSHIKAWA2, Eisuke DOMAE2, Aki NISHIURA3, and Naoyuki MATSUMOTO3

1Graduate School of Dentistry (Department of Orthodontics), Osaka Dental University
2Department of Biochemistry, Osaka Dental University
3Department of Orthodontics, Osaka Dental University

J Oral Tissue Engin 2020; 18(2): 55-62.

Objectives: Syndecans (SDCs) are a family of cell surface heparan sulfate proteoglycans known to play critical roles in regulating multiple cellular functions, including participation in inflammation and wound healing as a host defense molecule. The present study was undertaken to analyze the role of syndecan family in human dental pulp cells and the effect of an inflammatory cytokine.
Materials and methods: Human dental pulp cell cultures from impacted third molars were established. Cells were cultured with the inflammatory mediator interleukin-1beta (IL-1beta). The gene expressions of SDCs were analysed by quantitative RT-PCR.
Results and significance: The relative level of mRNA expressions for SDC1 and SDC2 increased with cell proliferation, but both of them were suppressed significantly by IL-1beta stimulation. The expression of SDC3 remained unaltered by IL-1beta, although the expression level for SDC4 showed a rapid and strong increase. SDC4 was shed into the culture medium in a process involving MMPs. Shedding of SDC4 was increased after IL-1beta-stimulation. Our data demonstrate that all of SDC family express in human dental pulp cells and play different roles, and, most notably, SDC4 is involved in acute inflammation.

Key words: dental pulp cells, inflammation, syndecans

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