Journal of Oral Tissue Engineering

Basic Studies of Carbon Nanotube/DNA- protamine Complex Membranes for Guided Bone Regeneration

Sayaka MORI-SHUTO3, Koichro MORI4,
Nozomi KAWASHIRI3, and Reira MINAMI3

1Department of Dental Bioengineering, Biomaterials Section, Fukuoka Dental College, Fukuoka, Japan
2Department of Odontology, Periodontology Section, Fukuoka Dental College, Fukuoka, Japan
3Department of Dental Hygiene, Fukuoka College of Health Sciences, Fukuoka, Japan
4Research Center for Regenerative Medicine, Fukuoka Dental College, Fukuoka, Japan

J Oral Tissue Engin 2020; 18(2): 63-70.

Carbon nanotubes (CNTs) release substantial vibrational energy after exposure to near-infrared (NIR) irradiation. This study examines the preparation of exothermic complexes comprising CNTs, DNA, and protamine (CNT/DNA-protamine complexes), and evaluates the complex membrane for use as a barrier membrane in guided bone regeneration (GBR). Heat generation of the CNT/DNA-protamine complex membrane increased with amount of CNTs in the complex, and the temperature increase reached 16-17.6°C after irradiation for 4 min. The degradation rate of the complex membrane immersed in PBS was influenced by the amount of CNTs and was lower than that of DNA-protamine complexes (DP complexes) used as a control. The CNT/DNA-protamine complexes showed higher resistance to compressive deformation than DP complexes. The results of this study suggested that CNT/DNA-protamine complexes may be candidate biodegradable mem-branes with controllable heat generation and an adjustable degradation rate for use in GBR.

Key words: carbon nanotubes, DNA, complex, barrier membrane, photothermal generation

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