Journal of Oral Tissue Engineering



ORIGINAL ARTICLE
Effects of Bicarbonated Ringer's Solution Heated to 37℃ on Human Periodontal Ligament Fibroblasts, Human Osteoblasts, and Human Dental Pulp-derived Fibroblasts

Tomoharu OKAMURA1, Tomoki TAKEUCHI1, Sho AOKI2, Suguru DATEOKA3,
Naoya UEMURA4, Hajime TABATA5, Harumi ISONO1, Jinhao CUI1, Yuma YAMAMOTO1, Chihoko IKEDA1,
Yoshiaki ONO3, Shunsuke BABA4, Makoto UMEDA5, and Kazuya TOMINAGA1

1Department of Oral Pathology, 2Department of Pediatric Dentistry,
3Department of Special Care Dentistry, 4Department of Oral Implantology, 5Department of Periodontology,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2021; 19(2): 41-51.

SYNOPSIS
In dental treatment, water is applied at room temperature during treatment to protect the cells from nociceptive stimulation by heat. In this study, the purpose was to confirm the use of bicarbonate Ringer's solution as an alternative to water in dental treatment. The main cells to be protected in dental treatment were preserved in water, saline and bicarbonate Ringer's solution for 60 minutes. Three different temperature conditions were used for storage. The results showed that for all liquid storage conditions, the temperature conditions closer to body temperature resulted in less cell death and higher mitochondrial activity. For the nutrient conditions, the closer the conditions were to body temperature, the less cell death and the higher the mitochondrial activity tended to be. The ability to survive more cells and maintain higher mitochondrial activity suggests the usefulness of bicarbonate Ringer's solution for dental treatment.

Key words: human periodontal ligament fibroblasts, human osteoblasts, human deciduous dental pulp-derived fibroblasts, homeostasis, bicarbonated Ringer's solution


All documents in this paper

J-Stage https://www.jstage.jst.go.jp/article/jarde/19/2/19_19_41/_article

DOI https://doi.org/10.11223/jarde.19.41