Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
Cell-adhesive Protein Immobilization Using Tresyl Chloride-activation Technique for the Enhancement of Initial Cell Attachment

Tohru HAYAKAWA1, Megumi NAGAI2, Masao YOSHINARI3, Msaharu MAKIMURA2, Kimiya NEMOTO1

1Department of Dental Materials, Research Institute of Oral Science,
Nihon University School of Dentistry at Matsudo,
2-870-1, Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan
2Department of Comprehensive Clinical Dentistry, Research Institute of
Oral Science, Nihon University School of Dentistry at Matsudo,
2-870-1, Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan
3Department of Dental Materials Science and Oral Health Science Center,
Tokyo Dental College, 1-2-2 Masago, Mihamaku, Chiba 261-8502, Japan

J Oral Tissue Engin 2004;2(1): 14-24

Full Text. DOI https://doi.org/10.11223/jarde.2.14

The aim of this study was to evaluate the biological effect of immobilized cell-adhesive protein onto titanium using tresyl chloride-activated technique. Titanium sputter-coated glass (Ti-glass) was used as a substrate. Tresyl chloride was directly applied onto Ti-glass. Fibronectin or collagen was immobilized to tresylated Ti-glass. The measurement of X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared reflection-adsorption spectroscopy confirmed the immobilization of fibronectin or collagen onto tresylated Ti-glass. The immobilization of fibronectin was not effective for enhancing the initial attachment of human gingival fibroblasts, but collagen immobilization was effective. The observation of scanning electron micro-scope also revealed that collagen immobilization affected the morphological changes of attached human fibroblast. In conclusion, the present study supported that colla-gen immobilization onto Ti-glass using tresyl chloride-activation technique improved the activity of human gingival fibroblasts during initial attachment.

Key words: fibronectin, collagen, fibroblast, tresyl chloride, titanium