Journal of Oral Tissue Engineering

Bone formation in titanium porous scaffold with immobilization of BMP-2

Kenichi MATSUZIKA1, Masao YOSHINARI2, Eitoyo KOKUBO1, Masaki SHIMONO3, Yasuo YAMADA4, Mamoru MABUCHI5 and Takashi INOUE1

1Department of Clinical Pathophysiology and Oral Health Science Center,
2Department of Dental Materials Science and Oral Health Science Center,
3Department of Pathology and Oral Health Science Center;
Tokyo Dental College; 1-2-2 Masago; Mihama-ku, Chiba 261-8502. Japan
4Materials Research Institute for Sustainable Development,
National Institute of Advanced Industrial Science and Technology (Chubu);
2266-98 Anagahora, Simoshidami, Moriyama-ku, Nagoya 463-8560, Japan
5Department of Energy Science and Technology,
Graduate School of Energy Science, Kyoto University,
Yoshidahonmachi, Sakyo, Kyoto 606-8501, Japan

J Oral Tissue Engin 2005;2(2): 60-65

Full Text. DOI

Objectives. Bone defects make up a significant clinical problem. The purpose of the present study was to evaluate the osteogenic tissue behavior in a titanium (Ti) porous scaffold with immobilization of bone morphogenetic protein (BMP) -2 in vivo.
Methods. Ti scaffold (pore size: 200-500 µm) with or without BMP-2 immobilization was implanted into a created defect of a rat femur.
Results. One week after implantation, only bone marrow cells were present around the scaffold without BMP, but new bone formation could be observed around the scaffold with BMP. Two weeks after implantation, new bone tissue had formed around the scaffold with and without BMP-2 immobilization.
Conclusions. Ti porous scaffold with BMP-2 immobilization can manufacture new bone tissue at an early stage, and can be a beneficial in the repair of bone defects.
Key words: Ti, porous, scaffold, BMP-2, O2-plasma treatment