Journal of Oral Tissue Engineering

Effects of strontium substituted hydroxyapatite of different porosities on osteoblasts in vitro

LIU Xue-yang and CHEN De-min

Ninth Peoplefs Hospital, Shanghai Second Medical University
Research and Testing Center for Biomaterials, Shanghai 200011, China

J Oral Tissue Engin 2005;2(2): 66-75

Full Text. DOI

Objectives: To compare the effects of strontium substituted hydroxyapatite of different porosities on the biological behaviors of the osteoblasts derived from rabbit bone marrow in in vitro culture.
Methods: Bone marrow was obtained from healthy rabbit,and then was cultured and in-duced into osteoblasts,which were compounded with Sr-HA and HA(as the positive con-trast) ceramics of different porosities(50%,60% and 70%).The proliferation and differen-tiation of all the cultured cells were observed at different time points under inverted phase contrast microscope and scanning electron microscope(SEM) respectively. The activity of alkaline phosphatase in these osteoblasts was quantitatively detected.
Results: After cultured with Sr-HA with different porosities, the osteoblasts from rabbit bone marrow showed the biological and morphological properties similar to those of un-compounded osteoblasts. Sr-HA didn't show any adverse effects on the cell functions. In addition, Sr-HA with a porosity of 70% could facilitate the entrance of cells into the pores of the material.
Conclusion: Sr-HA is an optimal scaffold material for bone tissue engineering, which can be potentially found clinical application for bone defect repair. Because of the good con-nectivity of Sr-HA with a porosity of 70%, it has better effect on the growth of osteoblasts and more possibility of application as a scaffold material for bone tissue engineering than Sr-HA with other porosities.

Key words: Strontium substituted hydroxyapatite, Porosity, Osteoblast, Biological behaviors