Journal of Oral Tissue Engineering

Effect of Fine Powdered Hybrid Resin Blocks for CAD/CAM Crowns on Rodent-derived Cells

Mariko NAKAI1, Yoshiya HASHIMOTO2, Junyi WANG2, Cheng TAN2, Yasuhiro MASUDA2,
Koichi IMAI3, and Nobutaka OKUSA1

1 Department of Forensic Dentistry, 2 Department of Biomaterials,
3 Department of Tissue Engineering, School of Dentistry,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2022; 20(2): 55-64.

Although fine dust is generated in large quantities during chairside adjustment and polishing of dental work during dental treatment, it may be hazardous to the health of patients, dentists, and dental staff. Previous studies on the fabrication of dust derived from dental materials and exposure to cultured cells have reported that lactate dehydrogenase and reactive oxygen species are released, causing cell cycle disruption. In this study, we fabricated dental CAD/CAM fine powders using a collagen-based three-dimensional (3D) culture method instead of the conventional two-dimensional (2D) culture method and evaluated cell viability in 2D and 3D cultures. Cell viability was evaluated by 2D and 3D cultures. Cell viability was also evaluated by measuring oxidative stress in 2D cultures. The results showed that exposure of cells to dust caused oxidative stress and the 3D culture method reduced cell viability, similar to the results for the 2D culture method. Adequate dust control measures should be taken in dental treatment rooms using extraoral vacuum, surgical masks, and gloves.

Key words: CAD/CAM, ROS, MTT, three-dimensional culture

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