Journal of Oral Tissue Engineering

Effect of an Inflammatory Mediator on the Syndecan Family Expression in Human Osteoblasts

Aiko KAMADA1, Yoshihiro YOSHIKAWA1, Isako SHIOJI1, Takashi IKEO1, Akiyo KAWAMOTO2, Kazutoshi KAKIMOTO2, Joji OKAZAKI2, Yutaka KOMASA2 and Chuanbin GUO3

1Department of Biochemistry, Osaka Dental University
2Department of Geriatric Dentistry, Osaka Dental University
3Department of Oral and Maxillofacial Surgery, School of Stomatology, Peking University, Beijing, China

J Oral Tissue Engin 2005;3(2): 105-111

Full Text. DOI

Bone cells are regulated by interaction with growth factors and components of the extracellular matrix. Syndecans are transmembrane heparan sulfate proteoglycans known to bind both other extracellular matrix molecules and certain growth factors. In this study using quantitative real-time RT-PCR, we investigated the expression of the syndecan family in normal human osteoblasts exposed to interleukin (IL)-1β as a bone resorptive agent. RT-PCR analysis demonstrated the mRNA expression of syndecan-1, -2 and -4 in normal human osteoblasts. When cells were incu-bated with IL-1β, there were significant decreases in mRNA levels for syndecan-1 and -2 at 24 h compared with time-matched controls. In contrast, the expression of syndecan-4 in IL-1β-stimulated osteoblasts markedly increased at 6 h followed by a decrease at 24 h. These results demonstrate that syndecan-1, -2 and -4 are synthesized in human osteoblasts, and syndecan-4 gene transcription is rapidly responsive to an inflammatory mediator.

Key words:syndecan, osteoblast, IL-1β