Journal of Oral Tissue Engineering

Epigallocatechin-3-O-gallate As a Novel Preservation Additive for Tooth Transplantation

Kazuaki MATSUMURA, Sadami TSUTSUMI and Suong-Hyu HYON

Institute for Frontier Medical Sciences,
Kyoto University, Japan

J Oral Tissue Engin 2005;3(3): 125-130

Full Text. DOI

The effects of green tea polyphenol, epigallocatechin-3-O-gallate (EGCG), on tooth preservation of guinea pig incisors were investigated.
Carefully extracted and pulpectomized teeth of the guinea pigs (200-300g, 4 weeks) were irrigated with saline. After rinsing in phosphate buffer saline containing 100U/mL of penicillin and 100 mg/mL of streptomycin, the teeth were transferred to Dulbecco's Modified Eagle's Medium (DMEM) containing 10 v/v% of fetal calf serum and different concentration of EGCg, followed by incubation at 4oC for one week. Then, the teeth were recovered and put on a culture dish with DMEM. Time until cell migration of EGCG treated teeth was shorter than that of untreated teeth.
The periodontal ligament cells viability preserved with 250 mg/mL EGCG at 4oC was higher than that without EGCG. These results suggested that EGCG could be useful as a cell protective agent at low temperature preservation.

Key words: Periodontal ligament, epigallocatechin-3-O-gallate, preservation, tooth transplantation