Journal of Oral Tissue Engineering

Influence of Hypothyroidism Induced by Thiamazole on the Toxicity of Fluconazole in Chick Embryos

Yuji YOSHIYAMA1, Katsuya OTORI2, Kazuo YAGO2 and Motoko KANKE1

1Division of Clinical Pharmacy, Kyoritsu University of Pharmacy, Japan
2Department of Pharmacy, Kitasato University Hospital, Japan

J Oral Tissue Engin 2006;4(1): 32-36

Full Text. DOI

The effect of hypothyroidism induced by thiamazole on the toxicity of fluconazole was studied in chick embryos. To our knowledge, no information is now available on the effects of thyroid gland function on the regeneration and differentiation of tissue. Fertilized eggs of White Leghorns were incubated and investigated. 1.2 mg/0.2 mL/egg of thiamazole was injected into the albumen of fertilized eggs on the 9th day of incubation. Fluconazole at 0.4 mg/egg was injected into the air sac of fertilized eggs on the 16th day of incubation. Electrocardiograms were recorded 0 to 60 min after the injection. After the injection of fluconazole into the thiama-zole-treated eggs, the heart rate was significantly decreased compared with the untreated eggs. These findings indicate that hypothyroidism induced by thiama-zole has a marked influence on the toxicity of fluconazole in chick embryos.

Key words: hypothyroidism; cardiotoxicity; chick embryo