Journal of Oral Tissue Engineering

Effect of Titanium Surface Roughness on the Cytocompatibility of Osteoblast-like Cells

Shimpei KAKEHI1, Shouji TAKEDA2 and Masaaki NAKAMURA2

1Graduate School of Dentistry (Biomaterials), Osaka Dental University, JAPAN, 2Department of Biomaterials, Osaka Dental University, JAPAN

J Oral Tissue Engin 2006;4(2): 77-88

Full Text. DOI

The aim of this study was to examine the effect of alumina blasted titanium disks, prepared by blasting with 7 different graded sizes of alumina particles, on osteoblast-like MG-63 cells. Following an evaluation of the surface conformation and the average surface roughness, the cell morphology, initial cell adhesion, cell proliferation, alkaline phosphatase (ALP) activity and osteocalcin (OC) production were examined. Both the cellular differentiation (ALP activity, OC) and initial cell attachment increased with a coarser surface. However, differences in surface roughness had no significant effect on cell proliferation.

Key words:titanium, implant, MG-63, sandblast