Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Effect of Acemannan on BMP-2 Expression in Primary Pulpal Fibroblasts And Periodontal Fibroblasts, in vitro Study

Nawaporn JITTAPIROMSAK1#, Suwimon JETTANACHEAWCHANKIT2#, Peechanika LARDUNGDEE3, Polkit SANGVANICH4 and Pasutha Din THUNYAKITPISAL5

1Dental Biomaterials Program, Faculty of Dentistry,
2Oral Biology Program, Faculty of Dentistry,
3Department of Pediatric, Faculty of Dentistry,
4Department of Chemistry, Faculty of Science,
5Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Thailand
# Equal distribution for this work

J Oral Tissue Engin 2007;4(3): 149-154
Full Text. DOI https://doi.org/10.11223/jarde.4.149
SYNOPSIS
Periodontal fibroblasts and pulpal fibroblasts have been considered as the major cells responsible for new alveolar bone and dentin formation, respectively. Bone morphogenic protein-2 (BMP-2) has great clinical potential for inducing bone and dentin repair and regeneration. The objective of this study was to investigate the effect of acemannan, polysaccharide fraction of aloe vera gel on BMP-2 expression in primary human periodontal fibroblasts and pulpal fibroblasts. Cells were treated with increasing concentration of acemannan for 24 and 48 hours. The reverse transcriptase-PCR and ELISA assays were used to investigate the effects of acemannan on the levels of BMP-2 mRNA and protein expression, respectively. The results from RT-PCR and ELISA revealed that acemannan induced BMP-2 mRNA level and protein synthesis in both cells. The effective concentrations of acemannan were 0.25-1 mg/ml. Collectively, these results suggest that acemannan is a potent plant polysaccharide for stimulating BMP-2 expression in periodontal fibroblasts and pulpal fibroblasts.

Key words: acemannan, BMP-2, periodontal fibroblasts, proliferation, pulpal fibroblasts