Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Effects on Porcine Mesenchymal Stem Cells When Cultured in Autologous Versus Fetal Bovine Serum

 Harutsugi ABUKAWA1, Arnulf BAUMANN2, Joseph P. VACANTI3, Leonard B. KABAN4 and Maria J.TROULIS5

1Department of Oral and Maxillofacial Surgery, Tokyo Medical University Hospital, Tokyo, Japan, 2Hospital of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, Vienna, Austria, 3Pediatric Surgery, Tissue Engineering and Organ Fabrication Laboratory, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA, 4Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital and Harvard School of Dental Medicine, Boston, MA, USA, 5Minimally Invasive Oral and Maxillofacial Surgery Program, Skeletal Biology Research Center, Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital and Harvard School of Dental Medicine, Boston, MA, USA
J Oral Tissue Engin 2007;5(1): 23-34
Full Text. DOI https://doi.org/10.11223/jarde.5.23
SYNOPSIS
The use of FBS (Fetal Bovine Serum) is accompanied by a risk of disease trans-mission. The aim of this study was to evaluate autologous serum (AS) as an alternative culture medium for tissue engineering. Alkaline phosphatase (ALP) activity, DNA production (cell proliferation), and production of mineralized bone matrix were assessed when porcine mesenchymal stem cells (pMSCs) were cultured in autologous versus fetal bovine serum. Cells were cultured as follows: 1) DMEM (Dulbecco's modified high glucose eagle's medium), osteogenic supplements (OS: 100 nM dexamethasone, 50µg/ml ascorbic acid, and 10 mM beta- glycerophosphate), and 10% active autologous serum (AAS); 2) DMEM, OS, and 10% inactivated (heated) autologous serum (IAS); 3) DMEM, OS, and 10% FBS (control). At 3, 7, 14 and 21 days in culture, Alkaline phosphatase (ALP) activity, DNA production (cell proliferation), and production of mineralized bone matrix were measured. ALP activity and DNA production were greater at all time points in the FBS (control) group versus the experimental (AAS and IAS) groups. However, ALP activity relative to DNA production in the AAS and IAS groups was equal to the FBS group.

Key words: autologous serum, autogenous serum, porcine, mesenchymal stem cells, differentiation