SYNOPSIS
In the present study, we attempted to mucosa reconstruction in an oral mucosa defect after removal of pleomorphic adenoma with the use of a cultured human oral epithelial cell sheet on human amniotic membrane (cell sheet), and obtained an excellent outcome.
The patient was a 45-year-old female, in whom a hard movable tumor with a clear boundary, of which the size was the index finger tip, was detected immediately below the left mucosa of her upper lip. The lesion was diagnosed with a benign tumor in the minor salivary gland, and its removal was decided. To reconstruct the oral mucosa following tumor surgery, we planned treatment using the human cell sheet, and the patient gave consent after explanation of the surgical method and evidence safety. This surgical treatment was approved by the Human Studies Committee of Kyoto Prefectural University of Medicine.
Punch biopsy was obtained for given oral epithelia, and produced a cell sheet by culturing the obtained oral epithelia on amniotic membrane for about 3 weeks. The cell sheet containing the amniotic membrane was sutured in the oral mucosa defect region, after removal of the tumor (10 x 7 mm), and fixed by the tie-over method. At present, about 3 months after surgery, neither contracture nor recurrence was detected, and the postoperative course was excellent.

Key words: Cultured human oral epithelial cell sheet, Human amniotic membrane, Pleomorphic adenoma, Oral Reconstruction