Journal of Oral Tissue Engineering

Pre-intercalated DNA/Lipid/PLGA Film as a Drug Carrier

Minoru KAWAGUCHI1, Tadao FUKUSHIMA1, Tohru HAYAKAWA2, Yusuke INOUE3 and Jun OHNO4

1Department of Dental Engineering, Bioengineering section,Fukuoka Dental College, Fukuoka, Japan
2Department of Dental Biomaterials, Research Institude of Oral Science,Nihon University School of Dentistry at Matsudo, Chiba, Japan
3Fukuoka College of Health Sciences, Fukuoka, Japan
4Department of Morphological Biology, Section of Pathology, Fukuoka Dental College, Fukuoka, Japan

J Oral Tissue Engin 2008;6(2): 97-105

Full Text. DOI

Complexes of daunorubicin hydrochloride (DH)-intercalated DNA and artificial lipids were synthesized. These DH-pre-intercalated DNA/lipid complexes were blended with PLGA (poly (D, L-lactide-co-glycolide)) to prepare DH-releasable films as a carrier material for a drug delivery system (DDS). The films could release DH in PBS solution. The amount of DH released was dependent on the chemical structure of the lipid and initial concentration of DH in the pre-intercalated DNA. Pre-intercalation slowed the release of DH better than post-intercalation. The DH released caused a reduction in cell viability during the culture of L-929 mouse fibroblasts. DH-pre-intercalated DNA/lipid/PLGA films have potential for the controlled-release of DH.

Key words: drug delivery system, DNA, artificial lipid, drug release