Journal of Oral Tissue Engineering

An Attempt to Cell Recovery Factor in the Cell Differentiation Culture with the Embryonic Stem Cell Test (EST)

Koichi IMAI1, Shinji KUSAKAWA2, Akito TANOUE2, Makiko KUWAGATA3, Mika SENUMA3, Mami FURUYA3, and Hiromasa TAKASHIMA3

1Department of Biomaterials, Osaka Dental University, Osaka, Japan,
2Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan,
3Laboratory of Toxicology, Hatano Research Institute, FDSC, Kanagawa, Japan

J Oral Tissue Engin 2008;6(3): 152-158

Full Text. DOI

The embryonic stem cell test (EST) is a validated in vitro assay that has been established to classify compounds with respect to their embryotoxic potential. In 1988, Imai has proposed the cell recovery test as one variation of cytotoxicity test. By exposing the material or medicine to cells, the transitory influence on recovery of the cell proliferation level is assessed. In that manner, the cell recovery test was also developed allowing the examination in not only recovery of cell proliferation but also recovery from chemical disruption of cell differentiation. After exposing both cells to NaF and SnF2 used to prevent tooth decay, we compared the results of each culture in fresh medium with the finding obtained by the EST.
In the non recovery conditions, NaF and SnF2 both showed "non embryotoxicity". The influence of cellular recovery was not demonstrated in NaF and SnF2. These chemicals are both fluorine compounds, the embryotoxicity level of SnF2 is presumed to be slightly stronger than that of NaF. Stronger embryotoxicity is a concern when the recovery culture shows that the toxicity level of a chemical remains constant. Therefore, it is necessary to consider the utility of examining recovery from embryotoxicity.

Key words: EST, embryotoxicity, cell recovery, NaF, SnF2