Journal of Oral Tissue Engineering

In Vitro New Capillary Formation with Eight Metal Ions of Dental Biomaterials

Koichi IMAI1, Tetsunari NISHIKAWA2, Akio TANAKA2, Kazuhiko SUESE3, Hiromasa TAKASHIMA4 and Shoji TAKEDA1

1Department of Biomaterials, 2Department of Oral Pathology,
Osaka Dental University, Osaka, Japan, 3Osaka Dental University,
School of Dental Technician and Hygienist, Osaka, Japan
4Hatano Research Institute, FDSC, Kanagawa, Japan

J Oral Tissue Engin 2010;8(1): 74-79

We evaluated the influences of the component ions (Ag, Au, Cu, In, Ni, Pd, Ti, and Zn) of dental alloys on the in vitro capillary formation of new blood vessels using a human angiogenesis kit (Kurabo). The percent area of new capillary in the presence of Zn ions was the highest, being 106.1% of that in the control group. The percent area decreased in the order of Ag, In, Ti, Ni, Cu, Pd, Au and Zn ions. The new capillary length was also the greatest for Zn ions and the smallest for Ag ions, but the order of the other types of metal ions differed from that of the percent area. Based on these results, if the release of a small amount of Zn ions can be controlled, the active rate of capillary formation may increase, resulting in earlier tissue regeneration.

Key words: angiogenesis, metal ion, tissue regeneration, dental, in vitro