Journal of Oral Tissue Engineering

Biological Reactions to Calcium Phosphate-coated Calcium Carbonate Particles

Tetsunari NISHIKAWA1, Kazuya MASUNO1, Tomoharu OKAMURA1, Kazuya TOMINAGA1, Masahiro WATO1, Mayu KOKUBU1, Koichi IMAI2, Shoji TAKEDA2, Yoichro TAGUCHI3, Masatoshi UEDA3 and Akio TANAKA1
1Department of Oral Pathology, 2Department of Biomaterials,
3Department of Periodontology, Osaka Dental University, Osaka Japan

J Oral Tissue Engin 2010;8(2): 115-123

Objectives: In order to histopathologically investigate biological reactions to materials used for scaffolds, we examined the cytotoxicity to calcium particles in vitro and bioabsorption in vivo. Materials and methods: Calcium phosphate-coated calcium carbonate particles 30µm in diameter (calcium particles) were used for the materials. In vitro; Normal human dermal fibroblasts (NHDF) and human umbilical vein endothelial cells were co-cultured and seed with calcium particles. In vivo; The calcium particles were used as the experimental group, and rat bone, mouse bone and propylene glycol alone serving as the control group were implanted under the dorsal skin of rats. Following transplantation at 2, 4, 6 and 8 weeks, the skins were resected. The sections stained with HE, DAPI, anti-rat CD3, anti-immunoglobulin, and anti-rat macrophage, were observed histopathologically. Results: The co-cultured cells grew densely around the calcium particles, and the latter were assimilated by granulation tissue comprised of CD3 dominant lymphocytes, macrophages, and foreign-body giant cells. The particles were phago-cytosed by foreign-body giant cells with 10-20 nuclei. The bioabsorption of the calcium particles was similar to that of rat bone, but different from that of mouse bone.

Key words: bioabsorption, lymphocytes, macrophages, calcium particles